Product Name: JNK2-3
Product Number: AB-NK189-4
Size: | 25 µg | | Price: | 89.00 |
| | | $US | |
Target Full Name: Jun N-terminus protein-serine kinase (stress-activated protein kinase (SAPK)) 2
Target Alias: c-Jun N-terminal kinase 2; JNK2alpha; JNK2-alpha-2; JNK2A; JNK2B; JNK2beta; JNK-55; Jun kinase; Kinase JNK2; MAPK9; MK09; p54a; PRKM9; Stress-activated protein kinase JNK2; JNK-55; SAPK1a; JNK2BETA; p54aSAPK; CCDS4453.1; ENSG00000050748
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK189-4
Antibody Target Type: Pan-specific
Protein UniProt: P45984 Protein SigNET: P45984 Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human JNK2 (MAPK9) sequence peptide Cat. No.: PE-01AXP90
Antibody Immunogen Sequence: MSDSKGDSQFYSVQVC
Antibody Immunogen Description: Corresponds to amino acid residues M1 to V15; N-terminus
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.25 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 48-54 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human JNK1 and JNK3.
Scientific Background: JNK2 (MAPK9) is a protein-serine/threonine kinase of the CMGC group and MAPK family. It is involved in the control of cell differentiation, transformation, migration, proliferation, and programmed cell death in response to stress and cytokines. It has also been implicated in preventing ubiquitination of p53.Phosphorylation of T183 and Y185 by either of two dual specificity kinases, MAP2K4 and MAP2K7, increases phosphotransferase activity. Phosphorylation of S129 also increases phosphotransferase activity. It is nhibited by dual specificity phosphatases, such as DUSP1, which target the T183 and Y185 activatory phosphorylation sites. JNK2 has been shown, in a cancer cell line to have a role in breast cancer cell motility.
Figure 1. Dot blotting JNK2-3 antibody with recombinant purified proteins.