Product Name: JNK3-4 (SAPKb-NT)
Product Number: AB-NK197
Size: 25 µg      Price:89.00
      $US
Target Full Name: Jun N-terminus protein-serine kinase (stress-activated protein kinase (SAPKb)) 3

Target Alias: c-Jun N-terminal kinase 3; FLJ12099; FLJ33785; JNK3A; JNK3-alpha-2; Kinase JNK3; MAP kinase p49 3F12; MAPK10; MGC50974; MK10; P493F12; p54bSAPK; PRKM10; Stress-activated protein kinase JNK3; PRKM10; CCDS3613.1; ENSG00000109339

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK197

Antibody Target Type: Pan-specific

Protein UniProt: P53779

Protein SigNET: P53779

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human JNK3 (MAPK10) sequence peptide

Antibody Immunogen Sequence: MSDSKSDGQFYSVQVAC

Antibody Immunogen Description: Corresponds to amino acid residues M1 to A16; N-terminus

Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: 100 mM Tris-glycine, pH 7.0

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Western blots with recombinant rat JNK3 expressed in E. coli.

Antibody Species Reactivity: Human; Mouse; Rat

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 48-54 kDa.

Scientific Background: JNK3 (MAPK10) is a protein-serine/threonine kinase of the CMGC group and MAPK family. Phosphorylation at T221 and Y223, by MAP2K4 and MAP2K7. MAP2K7, increases phosphotransferase activity. MAP2K7 phosphorylates JNK3 on T221 causing a conformational change and a large increase in Vmax. MAP2K4 then phosphorylates Y223 resulting in a further increase in Vmax. Phosphorylation at T131 inhibits phosphotransferase activity. It is inhibited by dual specificity phosphatases, such as DUSP1 and by HDAC9. The near absence of reported somatic mutations in this protein indicates that it may function as a tumour requiring protein (TRP) in many human tumours.
Figure 1. Dot blotting JNK3-4 antibody with recombinant purified proteins.