Product Name: ErbB3-1
Product Number: AB-NK231-2
Size: 25 µg      Price:89.00
      $US
Target Full Name: ErbB3 (HER3) receptor-tyrosine kinase

Target Alias: C-erbB3; ERB3; HER3; Kinase ErbB3; LCCS2; Receptor protein-tyrosine kinase erbB-3 precursor; Tyrosine kinase-type cell surface receptor HER3; V-erb-b2 erythroblastic leukemia viral oncogene 3; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; MGC88033; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3; ENSG00000065361; O75810; O75811; O75812; O75813; Q9BUD7; Q9NNX2; Q9NNX3

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK231-2

Antibody Target Type: Pan-specific

Protein UniProt: P21860

Protein SigNET: P21860

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human ErbB3 (HER3) sequence peptide Cat. No.: PE-01ARS99

Antibody Immunogen Sequence: DRDAEIVVKDNGRSC

Antibody Immunogen Description: Corresponds to amino acid residues D169 to C183; In the extracellular domain of the ErbB3 receptor.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Very strong immunoreactivity with recombinant human ErbB3 on protein dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 160-200 kDa.

Antibody Specificity: Medium
Scientific Background: ErbB3 (HER3) is a protein-tyrosine kinase of the TK group and EGFR family. It is a receptor kinase that bind and be activated by NTAK and neuregulins. It has been proposed to be deficient in phosphotransferase activity. While the kinase domain appararently lacks activity but heterodimerizes with other EGFRs permits transduction of growth signals. Phosphorylation of Y1222 and Y1260 induces interaction with PIK3C and PIK3R1. ErbB3 appears to be up-regulated in many cancer, including medulloblastomas, verrucous carcinomas, childhood medulloblastomas, carotid body tumours (CBT), and gestational trophoblastic tumours (GTD).
Figure 1. Dot blotting ErbB3-1 antibody with recombinant purified proteins.


Figure 2. Immunoblotting of various cell lines with NK231-2 antibody at a 500 X dilution. The position of the full size target protein ErbB3 is indicated. The expected molecular masses of ErbB3 isoforms range from148 to 20 kDa. Each lane was loaded with 20 µg of cell lysate protein.