Product Name: ErbB3-pY1307
Product Number: AB-PK617
Size: | 25 µg | | Price: | 89.00 |
| | | $US | |
Target Full Name: ErbB3 (HER3) receptor-tyrosine kinase
Target Alias: C-erbB3; ERB3; HER3; Kinase ErbB3; LCCS2; Receptor protein-tyrosine kinase erbB-3 precursor; Tyrosine kinase-type cell surface receptor HER3; V-erb-b2 erythroblastic leukemia viral oncogene 3; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; MGC88033; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3; ENSG00000065361; O75810; O75811; O75812; O75813; Q9BUD7; Q9NNX2; Q9NNX3
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK617
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y1307
Protein UniProt: P21860 Protein SigNET: P21860 Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ErbB3 (HER3) sequence peptide Cat. No.: PE-04AMD95
Antibody Immunogen Sequence: HVH(pY)ARL(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues H1304 to L1310; In the cytoplasmic region of ErbB3 near the C-terminus after the kinase catalytic domain. This is the major in vivo phosphorylation sites in ErbB3.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in A431 cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Scientific Background: ErbB3 (HER3) is a protein-tyrosine kinase of the TK group and EGFR family. It is a receptor kinase that bind and be activated by NTAK and neuregulins. It has been proposed to be deficient in phosphotransferase activity. While the kinase domain appararently lacks activity but heterodimerizes with other EGFRs permits transduction of growth signals. Phosphorylation of Y1222 and Y1260 induces interaction with PIK3C and PIK3R1. ErbB3 appears to be up-regulated in many cancer, including medulloblastomas, verrucous carcinomas, childhood medulloblastomas, carotid body tumours (CBT), and gestational trophoblastic tumours (GTD).
Figure 1. Epitope mapping of ErbB3-pY1307 antibody with similar phosphopeptides on dot blots.
Figure 2. Identification of phosphosites related to ErbB3-pY1307.