Product Name: ICK-pY156+pT157
Product Number: AB-PK655
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Intestinal cell (MAK-like) kinase
Target Alias: ECO; HICK; Intestinal cell (MAK-like) kinase; Intestinal cell kinase; KIAA0936; Kinase ICK; Laryngeal cancer kinase 2; LCK2; MAK-related kinase; MGC46090; MRK; Serine/threonine kinase ICK
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK655
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y156+T157
Protein UniProt: Q9UPZ9
Protein SigNET: Q9UPZ9
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Target Alias: ECO; HICK; Intestinal cell (MAK-like) kinase; Intestinal cell kinase; KIAA0936; Kinase ICK; Laryngeal cancer kinase 2; LCK2; MAK-related kinase; MGC46090; MRK; Serine/threonine kinase ICK
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK655
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y156+T157
Protein UniProt: Q9UPZ9
Protein SigNET: Q9UPZ9
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ICK sequence peptide Cat. No.: PE-04ABV99
Antibody Immunogen Sequence: SKPP(pY)(pT)D(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues S152 to D158; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Immunogen Sequence: SKPP(pY)(pT)D(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues S152 to D158; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 64-67 kDa.
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 64-67 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431, HEK-293, and HeLa cells, or in sea star oocytes, except a medium cross-reactive 80 kDa protein in sea star oocytes.
Related Product 1: ICK-pY156+pT157 blocking peptide
Related Product 2: ICK-pY156+pT157 phosphosite-specific antibody (Cat. No.: AB-PK655)
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431, HEK-293, and HeLa cells, or in sea star oocytes, except a medium cross-reactive 80 kDa protein in sea star oocytes.
Related Product 1: ICK-pY156+pT157 blocking peptide
Related Product 2: ICK-pY156+pT157 phosphosite-specific antibody (Cat. No.: AB-PK655)
Scientific Background: ICK is a protein-serine/threonine kinase of the CMGC group and RCK family. It displays sequence similarity to the MAP kinases in the catalytic domain and the requirement for dual phosphorylation for activation within a MAP kinase-like TDY motif. ICK functions in the regulation of proliferation and differentiation in intestinal cells. Impairing ICK function has been shown to dowregulate the translation of specific targets of eIF4E and S6K1 (e.g. cyclin D1 and c-Myc) mediated through the mTOR/Raptor signalling pathway. Additionally, ICK homologuess in algae and invertebrates function in the formation of flagella and in ciliogenesis.
Figure 1. Dot blotting ICK-pY156+pT157 antibody with recombinant purified proteins.
Figure 2. Epitope mapping of ICK-pY156+pT157 antibody with similar phosphopeptides on dot blots.
Figure 3. Identification of phosphosites related to ICK-pY156+pT157.
© Kinexus Bioinformatics Corporation 2017