Target Full Name: Gamma-aminobutyric acid receptor subunit alpha-2

Target Alias: GABA A receptor subunit alpha 2; GABRA2; Gamma aminobutyric acid A receptor Alpha 2; GBRA2_Human

Product Type Specific: Chloride channel pan-specific antibody

Antibody Code: NN250-1

Antibody Target Type: Pan-specific

Protein UniProt: Q8TBI4

Protein SigNET: Q8TBI4

Antibody Type: Monoclonal

Antibody Host Species: Mouse

Antibody Ig Isotype Clone: IgG1

Antibody Immunogen Source: Fusion protein amino acids 350-385 (Cytoplasmic C-terminus) of rat GABA-A Receptor Alpha2

Production Method: Protein G purified

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence

Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.

Antibody Potency: In mouse brain lysates, this antibody weakly detects a ~55 kDa protein by Western blotting.

Antibody Species Reactivity: Mouse | Rat

Antibody Positive Control: A 1:100 dilution of SMC-486 was sufficient for detection of GABA-A R, Alpha2 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Antibody Specificity: Very high

Antibody Cross Reactivity: Does not cross-react with GABA-A Receptor Alpha1.

Related Product 1: GABA-A Receptor Alpha4 pan-specific antibody (Cat. No.: AB-NN251-1)

Related Product 2: GABA-A Receptor Alpha5 pan-specific antibody (Cat. No.: AB-NN252-1)