Product Name: GABA-A Receptor Alpha4
Product Number: AB-NN251-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Gamma-aminobutyric acid receptor subunit alpha-4
Target Alias: GABA A receptor subunit alpha 4; GABRA4; Gamma aminobutyric acid A receptor Alpha 4
Product Type Specific: Chloride channel pan-specific antibody
Antibody Code: NN251-1
Antibody Target Type: Pan-specific
Protein UniProt: P48169
Protein SigNET: P48169
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 410-450 (Cytoplasmic C-terminus) of rat GABA-A-Receptor Alpha 4
Target Alias: GABA A receptor subunit alpha 4; GABRA4; Gamma aminobutyric acid A receptor Alpha 4
Product Type Specific: Chloride channel pan-specific antibody
Antibody Code: NN251-1
Antibody Target Type: Pan-specific
Protein UniProt: P48169
Protein SigNET: P48169
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 410-450 (Cytoplasmic C-terminus) of rat GABA-A-Receptor Alpha 4
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~60 kDa protein by Western blotting.
Antibody Species Reactivity: Mouse | Rat
Antibody Positive Control: A 1:100 dilution of SMC-489 was sufficient for detection of GABA-A R, Alpha4 in 2 0µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross-react with other GABA-A-Receptor Alpha subunits.
Related Product 1: GABA-A Receptor Alpha2 pan-specific antibody (Cat. No.: AB-NN250-1)
Related Product 2: GABA-A Receptor Alpha5 pan-specific antibody (Cat. No.: AB-NN252-1)
Antibody Species Reactivity: Mouse | Rat
Antibody Positive Control: A 1:100 dilution of SMC-489 was sufficient for detection of GABA-A R, Alpha4 in 2 0µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross-react with other GABA-A-Receptor Alpha subunits.
Related Product 1: GABA-A Receptor Alpha2 pan-specific antibody (Cat. No.: AB-NN250-1)
Related Product 2: GABA-A Receptor Alpha5 pan-specific antibody (Cat. No.: AB-NN252-1)
Related Product 3: GABA-A Receptor Beta1 pan-specific antibody (Cat. No.: AB-NN253-1)
Related Product 4: GABA-A Receptor Beta3 pan-specific antibody (Cat. No.: AB-NN254-1)
Related Product 5: GABA-A Receptor Epsilon pan-specific antibody (Cat. No.: AB-NN255-1)
Related Product 6: GABA-B Receptor 1 pan-specific antibody (Cat. No.: AB-NN259-1)
Related Product 7: GABA-B Receptor 2 pan-specific antibody (Cat. No.: AB-NN256-2)
Related Product 4: GABA-A Receptor Beta3 pan-specific antibody (Cat. No.: AB-NN254-1)
Related Product 5: GABA-A Receptor Epsilon pan-specific antibody (Cat. No.: AB-NN255-1)
Related Product 6: GABA-B Receptor 1 pan-specific antibody (Cat. No.: AB-NN259-1)
Related Product 7: GABA-B Receptor 2 pan-specific antibody (Cat. No.: AB-NN256-2)
Scientific Background: The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2). GABA-A Receptor Alpha 4 is associated with epilepsy (3), and research suggests that alterations in GABA- A Receptors subtypes may be involved in the resistance to antiepileptic drugs (4).
References
[1] Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136.
[2] Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602.
[3] Grabenstatter H.L. et al. (2014) Epilepsia. 55(11): 1826-1833.
[4] Bethmann K., Fritschy J.M., Brandt C., Loscher W. (2008) Neurbiol Dis. 31(2): 169-187.
[1] Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136.
[2] Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602.
[3] Grabenstatter H.L. et al. (2014) Epilepsia. 55(11): 1826-1833.
[4] Bethmann K., Fritschy J.M., Brandt C., Loscher W. (2008) Neurbiol Dis. 31(2): 169-187.
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