Product Name: GABA-A Receptor Alpha5
Product Number: AB-NN252-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Gamma-aminobutyric acid receptor subunit alpha-5
Target Alias: Gamma-aminobutyric acid receptor subunit alpha-5; GABA(A) Receptor subunit Alpha-5; Gabra5
Product Type Specific: Chloride channel pan-specific antibody
Antibody Code: NN252-1
Antibody Target Type: Pan-specific
Protein UniProt: P31644
Protein SigNET: P31644
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Target Alias: Gamma-aminobutyric acid receptor subunit alpha-5; GABA(A) Receptor subunit Alpha-5; Gabra5
Product Type Specific: Chloride channel pan-specific antibody
Antibody Code: NN252-1
Antibody Target Type: Pan-specific
Protein UniProt: P31644
Protein SigNET: P31644
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 368-419 (last cytoplasmic domain before extracellular C-terminus) of human GABA-A Receptor Alpha5.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~55 kDa protein by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: A 1:100 dilution of SMC-494 was sufficient for detection of GABA-A R, Alpha5 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Weak ~60 kDa protein that may be the target in mouse brain lysates. Does not cross-react with GABA-A Receptor Alpha2 or other GABA-A Receptors based on KO validation data.
Antibody Potency: In mouse brain lysates, this antibody detects a ~55 kDa protein by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: A 1:100 dilution of SMC-494 was sufficient for detection of GABA-A R, Alpha5 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Weak ~60 kDa protein that may be the target in mouse brain lysates. Does not cross-react with GABA-A Receptor Alpha2 or other GABA-A Receptors based on KO validation data.
Related Product 1: GABA-A Receptor Alpha2 pan-specific antibody (Cat. No.: AB-NN250-1)
Related Product 2: GABA-A Receptor Alpha4 pan-specific antibody (Cat. No.: AB-NN251-1)
Related Product 3: GABA-A Receptor Beta1 pan-specific antibody (Cat. No.: AB-NN253-1)
Related Product 4: GABA-A Receptor Beta3 pan-specific antibody (Cat. No.: AB-NN254-1)
Related Product 5: GABA-A Receptor Epsilon pan-specific antibody (Cat. No.: AB-NN255-1)
Related Product 6: GABA-B Receptor 1 pan-specific antibody (Cat. No.: AB-NN259-1)
Related Product 7: GABA-B Receptor 2 pan-specific antibody (Cat. No.: AB-NN256-2)
Related Product 2: GABA-A Receptor Alpha4 pan-specific antibody (Cat. No.: AB-NN251-1)
Related Product 3: GABA-A Receptor Beta1 pan-specific antibody (Cat. No.: AB-NN253-1)
Related Product 4: GABA-A Receptor Beta3 pan-specific antibody (Cat. No.: AB-NN254-1)
Related Product 5: GABA-A Receptor Epsilon pan-specific antibody (Cat. No.: AB-NN255-1)
Related Product 6: GABA-B Receptor 1 pan-specific antibody (Cat. No.: AB-NN259-1)
Related Product 7: GABA-B Receptor 2 pan-specific antibody (Cat. No.: AB-NN256-2)
Scientific Background: The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2). Recent research shows their usage in investigating side effects of drugs and the effects of learning and memory. Inverse agonists of the Alpha5 subunit may be useful in the treatment of Alzheimer's Disease (3).
References
[1] Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136.
[2] Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602.
[3] Chambers M.S. et al. (2002) J Med Chem. 45(6): 1176-1179.
[1] Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136.
[2] Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602.
[3] Chambers M.S. et al. (2002) J Med Chem. 45(6): 1176-1179.
© Kinexus Bioinformatics Corporation 2017